Hi the gatk team,
I used HaplotypeCaller to generate a vcf file. Everything went fine : I got no error message, the vcf.gz and the vcf.gz.tbi were generated , however, the exit status was not '0' but 141
Dec 11, 2019 1:03:37 PM shaded.cloud_nio.com.google.auth.oauth2.ComputeEngineCredentials runningOnComputeEngine INFO: Failed to detect whether we are running on Google Compute Engine. 13:03:37.743 INFO HaplotypeCaller - ------------------------------------------------------------ 13:03:37.744 INFO HaplotypeCaller - The Genome Analysis Toolkit (GATK) v4.1.4.1 13:03:37.744 INFO HaplotypeCaller - For support and documentation go to https://software.broadinstitute.org/gatk/ 13:03:37.745 INFO HaplotypeCaller - Executing as lindenbaum-p@gkq0xd2.compute.bird2.prive on Linux v3.10.0-957.21.3.el7.x86_64 amd64 13:03:37.745 INFO HaplotypeCaller - Java runtime: Java HotSpot(TM) 64-Bit Server VM v1.8.0_131-b11 13:03:37.746 INFO HaplotypeCaller - Start Date/Time: December 11, 2019 1:03:36 PM CET 13:03:37.746 INFO HaplotypeCaller - ------------------------------------------------------------ 13:03:37.746 INFO HaplotypeCaller - ------------------------------------------------------------ 13:03:37.749 INFO HaplotypeCaller - HTSJDK Version: 2.21.0 13:03:37.750 INFO HaplotypeCaller - Picard Version: 2.21.2 13:03:37.750 INFO HaplotypeCaller - HTSJDK Defaults.COMPRESSION_LEVEL : 2 13:03:37.750 INFO HaplotypeCaller - HTSJDK Defaults.USE_ASYNC_IO_READ_FOR_SAMTOOLS : false 13:03:37.750 INFO HaplotypeCaller - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_SAMTOOLS : true 13:03:37.750 INFO HaplotypeCaller - HTSJDK Defaults.USE_ASYNC_IO_WRITE_FOR_TRIBBLE : false 13:03:37.751 INFO HaplotypeCaller - Deflater: IntelDeflater 13:03:37.751 INFO HaplotypeCaller - Inflater: IntelInflater 13:03:37.751 INFO HaplotypeCaller - GCS max retries/reopens: 20 13:03:37.751 INFO HaplotypeCaller - Requester pays: disabled 13:03:37.751 INFO HaplotypeCaller - Initializing engine (...) 13:28:24.643 INFO HaplotypeCaller - Shutting down engine [December 11, 2019 1:28:24 PM CET] org.broadinstitute.hellbender.tools.walkers.haplotypecaller.HaplotypeCaller done. Elapsed time: 24.81 minutes. Runtime.totalMemory()=1274019840 Using GATK jar /sandbox/apps/bioinfo/binaries/gatk/0.0.0/gatk-4.1.4.1/gatk-package-4.1.4.1-local.jar Running: java -Dsamjdk.use_async_io_read_samtools=false -Dsamjdk.use_async_io_write_samtools=true -Dsamjdk.use_async_io_write_tribble=false -Dsamjdk.compression_level=2 -Djava. io.tmpdir=. -Xmx5g -jar /path/to/gatk-package-4.1.4.1-local.jar HaplotypeCaller --tmp-dir . --reference /path/to/ref.fasta -I /path/to/input.bam -L <my-interval> -O out.g.vcf.gz --dbsnp path/to/dbsnp_135.b3 7.vcf.gz --do-not-run-physical-phasing --emit-ref-confidence GVCF
the vcf is ok
$ bcftools view out.g.vcf.gz > /dev/null && echo ok ok $ bcftools view out.g.vcf.gz 22 > /dev/null && echo ok ok
is there anything I can do to know about this error ? My FS is quite slow those days, is there any timeout to unlock some files ?