Quantcast
Channel: Recent Discussions — GATK-Forum
Viewing all articles
Browse latest Browse all 12345

VariantFiltration and BaseRecalibrator

$
0
0

So I've created a pipeline to make a known.snp.vcf for my species without known snps for BaseRecalibrator:

For a subset of my samples (~5% of my dataset, 10 samples):
I ran HaplotypeCaller on each bam file -> GenomicDBImport -> GenotypeVCFs -> SelectVariants SNP -> VariantFiltration.

My questions are:
1) is that a fair practice to estimate known SNPs?

2) Can I use that filtered vcf for BaseRecalibrator on my remaining samples? Will BaseRecalibrator recognize the PASS/FAIL and just use the PASS snps or do I need to take another step to weed out the poor quality snps?


Viewing all articles
Browse latest Browse all 12345

Trending Articles



<script src="https://jsc.adskeeper.com/r/s/rssing.com.1596347.js" async> </script>