I need to produce a set of all DNA-level variants for cancer patients to serve as exclusion sites for RNA editing site finding. Currently have have matched Tumor-Normal WES data for these patients. Which of the following would be the most appropriate way to produce these?
- Unfiltered paired MuTect2
- HC of NORMAL plus filtered paired MuTect2, or
- HC of TUMOR plus filtered paired MuTect2?