Hello,
We found a mutation was tagged as "PASS" in FILTER field with mutect2 (3.6 version) for some samples, however, this mutation is in our Panel of normal. For some other samples, this mutation was tagged as "panel_of_normals" correctly with the same version of mutect2. How can we explain why a PON mutation was tagged as "PASS" sometimes?
Let me know if you need any details information.
Rebecca